产品说明    Type I-A型，活性≥50 Kunitz units/mg 蛋白       保存：−20°C

Type I-A, powder, ≥60% as RNase A (SDS-PAGE), ≥50 Kunitz units/mg protein 
RNase A  核糖核酸酶A 

Synonym 

Pancreatic Ribonuclease

Ribonuclease I

Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

RNase A

Descriptions

Analysis Note  

Protein determined by E1%/280.

Application  

• RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.

Preparation Note  

Salt fractionated and chromatographically purified.

Properties

type   Type I-A

assay    ≥60% as RNase A (SDS-PAGE)

form    powder

mol wt    mol wt ~13,700

total impurities     salt, essentially free

foreign activity    protease, essentially free

storage temp.    −20°C

Safety

Safety Statements    22-24/25

WGK Germany   3

RTECS   RF0760000

F  10-21