Concanavalin A   刀豆蛋白A

PB10186-1 Sigma-Aldrich原 C2010 100mg 680.00

PB10186-2 Sigma-Aldrich原 C2010 1g 3356.00

产品说明     活性：＞500U/mg,purified 蛋白含量约15%（Biuret）   保存：-20℃   

Concanavalin A刀豆蛋白A

Synonym      Con A

CAS Number   11028-71-0

MDL number   MFCD00071069 

Descriptions

Analysis Note  

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Biochem/physiol Actions  

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Packaging  

Package size based on protein content

Preparation Note  

Purified by affinity chromatography.

Physical form  

Lyophilized powder containing NaCl

Properties

type        Type III

form        lyophilized powder

potency      <500 μg per mL agglutination activity

composition       Protein, ~15% biuret

total impurities     Carbohydrate, essentially free

storage temp.     2-8°C

Safety

Hazard Codes      Xn

Risk Statements      42/43

Safety Statements    24/25-36

WGK Germany    3

RTECS      GK6890000