检测方法     荧光法（530nm/590nm）测定细胞活性、细胞增殖、细胞毒性。

产品说明   该匀相检测法只需将一种工作试剂加至细胞培养液中，培养后即可测定荧光强度（激发波长= 530 - 570 nm，发射波长= 590 - 620 nm）。该试剂与其它基于刃天青的检测法（如Alamar Blue试剂）相同，采用的是氧化还原显色剂刃天青，刃天青本身并没有荧光性，但一旦被具有代谢活性的细胞还原，则会转化生成一种强荧光产物（试卤灵）。活细胞很容易还原这种无毒试剂，从而使荧光强度升高，因此可用荧光分光光度计或荧光分析仪方便地检测。非活性细胞没有代谢能力，因此不能还原该显色剂。所以检测中观察到的荧光强度能够准确检测活性细胞的数量。本公司生产的试剂经优化处理，提高了敏感性、可重复性、稳定性。这种基于细胞的匀相检测法可在多孔板中完成。该试剂与所有培养基以及所有液体处理系统都兼容，可在96孔板和384孔板中进行高通量筛选。适用范围包括细胞增殖、细胞毒性和细胞凋亡。

产品特点     安全：非放射性检测（参见³H-胸腺嘧啶核苷掺入法检测）。敏感且准确：可准确定量低至100个细胞。高效：采用96孔板和384孔板进行高通量检测，每天可同时处理成千上万的样品。匀相且简单：只需加入一种试剂的“混合-培养-测量”型检测法。无需冲洗或试剂移取步骤。高通量：在96孔板和384孔板中观察到的Z’系数通常为0.6-0.9。采用高通量筛选液体处理系统该检测易于自动化。 

适用范围     细胞增殖: 细胞因子、生长因子和营养成分对活体细胞的影响。细胞毒性和细胞凋亡: 评价有毒化合物、抗癌抗体、 毒素和环境污染物等。药物鉴定: 高通量筛选抗癌药物。

储存条件： 在4°C下保存。

Description  This homogeneous assay involves simply adding a single reagent, the CellQuanti-Blue^TM reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 - 570 nm, emission wavelength = 590 - 620 nm) after an incubation step. The CellQuanti-Blue^TM reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The CellQuanti-Blue^TM reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.

Key features  Safe. Non-radioactive assay (cf. ³H-thymidine incorporation assay).  Sensitive and accurate. As low as 100 cells can be accurately quantified. Time efficient. High-throughput assay in 96-well and 384-well plates allows simultaneous processing ten of thousands of samples per day. Homogeneous and convenient. A single reagent and "mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z’ factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.  

Applications  Cell Proliferation: effects of cytokines, growth factor, nutrients. Cytotoxicity and Apoptosis: evaluation of toxic compounds, anti-cancer antibodies, toxins, environmental pollutants etc. Drug Discovery: high-throughput screening for anticancer drugs.

Storage conditions. The CellQuanti-Blue^TM reagent is light sensitive and should be stored in the provided amber bottle at 4°C. The Control Reagent is stable at -20°C. Shelf life: 12 months.

编号：CQBL-048, CQBL-05K

测试：48T, 5000T