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溶壁酶(lywallzyme

<span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶使用说明书 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">  货号:BD-Lywallzyme 01 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">英文名称：Lywallzyme <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">其他名称：真菌细胞壁溶解酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶活性：原生质体产率≥1.0×106 个/mL<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。

￥1350.00

销量
0

评论
0

库存
99999
头孢唑林钠Cefazolin

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt

￥45.00

销量
0

评论
0

库存
2000
MES 2-(N-吗啡啉)乙

MES 2-(N-吗啡啉)乙磺酸 CAS号 145224-94-8  分子式 C6H13NO4S·H2O 分子量213.25

￥80.00

销量
0

评论
0

库存
3000
Microbank菌种冻存管

PL.170 菌种冻存管Microbank

￥1600.00

销量
0

评论
0

库存
10
TRIzol Plus RN

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Plus RNA Purification Kit <img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///https://assets.thermofisher.com/tfs-assets/lsg/product-images/12183555_650x600.jpg-250.jpg"/> Catalog number: 12183555 

￥3152.00

销量
0

评论
0

库存
100

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生化试剂

有关研究的生物材料

分子生物学
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[试剂]
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[thermo-试剂，内切酶]
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[thermo-试剂，内切酶]
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[细胞生物学]
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1F 试剂

ELISA -CIoud-clone corp

研究所用耗材

2F 耗材

耗材

BIOplastics 荧光定量PCR耗材

哈迈色谱样品瓶

荧光定量PCR耗材
常用国产耗材
常用-进口耗材

[试剂]
溶壁酶(lywallzy...

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color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。
[常用生化试剂]
头孢唑林钠Cefazol...

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt
[常用生化试剂]
MES 2-(N-吗啡啉...

2019-10-29

市场价: ￥80.00

价格: ￥80.00

MES 2-(N-吗啡啉)乙磺酸 CAS号 145224-94-8  分子式 C6H13NO4S·H2O 分子量213.25
[常用进口耗材]
Microbank菌种冻...

2019-03-13

市场价: ￥1600.00

价格: ￥1600.00

PL.170 菌种冻存管Microbank
[试剂]
TRIzol Plus ...

2019-01-25

市场价: ￥3708.00

价格: ￥3152.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Plus RNA Purification Kit <img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///https://assets.thermofisher.com/tfs-assets/lsg/product-images/12183555_650x600.jpg-250.jpg"/> Catalog number: 12183555
[生化试剂]
TRIzol Reage...

2019-01-25

市场价: ￥2637.00

价格: ￥1978.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>  TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Reagent

[常用进口耗材]
Microbank菌种冻...

2019-03-13

市场价: ￥1600.00

价格: ￥1600.00

PL.170 菌种冻存管
[常用进口耗材]
PALL滤器0.45um...

2018-01-09

市场价: ￥550.00

价格: ￥375.00

<p class="tp3" style="font-size: 12px; list-style-type: none; padding-right: 3px; padding-left: 3px; font-family: 微软雅黑; width: 120px; line-height: 25px; float: left; color: rgb(102, 102, 102); word-wrap: break-word; white-space: normal;">针头过滤器
[常用进口耗材]
紫外线防护眼镜

2017-12-23

市场价: ￥260.00

价格: ￥160.00

<h3 class="tb-main-title" data-title="HS-30紫外线防护眼镜实验室防护眼镜紫外灯眼镜 UV灯防紫外眼镜" data-spm-anchor-id="2013.1.iteminfo.i0.6e7ff689zwrlPm" style="margin-top: 0px; margin-bottom: 0px; font-size: 16px; min-height: 21px; line-height: 21px; color: rgb(60, 60, 60); font-family: tahoma, arial, " hiragino="" sans="" white-space:="" background-color:="">紫外线防护眼镜实验室防</h3>
[常用进口耗材]
程序降温盒

2017-12-23

市场价: ￥550.00

价格: ￥480.00

<h3 class="tb-main-title" data-title="Nalgene 程序降温盒5100-0001 梯度降温冻存盒" data-spm-anchor-id="2013.1.iteminfo.i0.6fefa80caGf89N" style="margin-top: 0px; margin-bottom: 0px; font-size: 16px; min-height: 21px; line-height: 21px; color: rgb(60, 60, 60); font-family: tahoma, arial, " hiragino="" sans="" white-space:="" background-color:="">Nalgene 程序降温</h3>
[常用进口耗材]
电击杯

2018-11-20

市场价: ￥80.00

价格: ￥55.00

 <h3 class="t
[常用进口耗材]
超滤离心管15ml

2018-11-20

市场价: ￥105.00

价格: ￥95.00

<ul class=" list-paddingleft-2" style="color: rgb(0, 0, 0); font-size: 12px; white-space: normal; font-family: tahoma, arial, "><li>品牌: 密理博</li></ul>

3F 仪器及维修

原装进口

正品保证感恩回馈

PCR仪-博日
超声仪-宁波新芝

[试剂]
溶壁酶(lywallzy...

2022-12-20

市场价: ￥1350.00

价格: ￥1350.00

<span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶使用说明书 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">  货号:BD-Lywallzyme 01 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">英文名称：Lywallzyme <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">其他名称：真菌细胞壁溶解酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶活性：原生质体产率≥1.0×106 个/mL<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。
[常用生化试剂]
头孢唑林钠Cefazol...

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt
[常用生化试剂]
MES 2-(N-吗啡啉...

2019-10-29

市场价: ￥80.00

价格: ￥80.00

MES 2-(N-吗啡啉)乙磺酸 CAS号 145224-94-8  分子式 C6H13NO4S·H2O 分子量213.25
[常用进口耗材]
Microbank菌种冻...

2019-03-13

市场价: ￥1600.00

价格: ￥1600.00

PL.170 菌种冻存管Microbank
[试剂]
TRIzol Plus ...

2019-01-25

市场价: ￥3708.00

价格: ￥3152.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Plus RNA Purification Kit <img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///https://assets.thermofisher.com/tfs-assets/lsg/product-images/12183555_650x600.jpg-250.jpg"/> Catalog number: 12183555
[生化试剂]
TRIzol Reage...

2019-01-25

市场价: ￥2637.00

价格: ￥1978.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>  TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Reagent

超声仪-宁波新芝

电泳仪-北京六一

PCR仪-博日

微生物学
生化试剂
有机溶液标准物质

[试剂]
溶壁酶(lywallzy...

2022-12-20

市场价: ￥1350.00

价格: ￥1350.00

<span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶使用说明书 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">  货号:BD-Lywallzyme 01 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">英文名称：Lywallzyme <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">其他名称：真菌细胞壁溶解酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶活性：原生质体产率≥1.0×106 个/mL<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">酶性状：灰白色或浅棕色粉末，易溶于水，活性稳定，较耐高温，便于贮存，产品质量稳定。 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">适用范围：酶解真菌细胞壁。对大多数真菌都有较好的脱壁效果，对担子菌和子囊菌的效果<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">*佳。其中食用和药用真菌，如草菇、平菇、蘑菇、香菇、银耳和灵芝等；工农业生产上的<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">重要菌种，如产黄青霉、黑曲霉、酵母等；研究遗传用的重要材料，如裂褶菌、粗糙脉孢菌、<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">构槽曲霉等，都能用溶壁酶去壁，获得大量原生质体，分离的原生质体活性强，有再生能力。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">用途：分解真菌细胞壁，可获得高产、优质的原生质体。该酶是真菌生物工程研究的有效工<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">具酶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">保存方法：3 个月以内：4℃干燥保存；3 个月以上：-20℃干燥保存。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">包装规格：1 克/瓶 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">溶壁酶酶解真菌菌丝获得原生质体 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">参考方法 <br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">一、材料<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、供试菌株：凤尾菇（Pleurotus sajor-caju）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、培养基：PD-马铃薯液体培养基。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、稳渗剂：0.6mol/LMgSO4 溶液或 0.8mol/L 甘露醇溶液。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、酶液：用稳渗剂配置含溶壁酶 15mg/mL 的酶液 3mL。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">二、方法<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1、菌丝培养：供试菌株斜面种转入马铃薯液体培养基中，25℃恒温静置培养 6 天。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">2、菌丝处理：将培养好的菌丝用无菌纯水洗涤 2 次。再用稳渗剂洗涤 1 次，离心去水<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">分。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">3、酶解：取处理好的菌丝 300mg 至配好的酶液中，摇匀，置 30℃恒温水浴中水解反应<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">1.5h，反应过程间歇振摇（动作应温和）。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">4、原生质体收集：酶解结束后，酶解液用灭菌脱脂棉过滤。滤液于 2000rpm 离心 10min，<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">收集原生质体。<br style="box-sizing: border-box; color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; white-space: normal; background-color: rgb(255, 255, 255);"/><span style="color: rgb(100, 100, 100); font-family: -apple-system, BlinkMacSystemFont, "Segoe UI", "PingFang SC", "Hiragino Sans GB", "Microsoft YaHei", "Helvetica Neue", Helvetica, Arial, sans-serif, "Apple Color Emoji", "Segoe UI Emoji", "Segoe UI Symbol"; font-size: 14px; background-color: rgb(255, 255, 255);">5、原生质体计数：离心完成后弃上清液，沉淀即为原生质体。原生质体用稳渗剂定至 3mL，然后用血球计数法计算原生质体量。
[常用生化试剂]
头孢唑林钠Cefazol...

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt
[常用生化试剂]
MES 2-(N-吗啡啉...

2019-10-29

市场价: ￥80.00

价格: ￥80.00

MES 2-(N-吗啡啉)乙磺酸 CAS号 145224-94-8  分子式 C6H13NO4S·H2O 分子量213.25
[常用进口耗材]
Microbank菌种冻...

2019-03-13

市场价: ￥1600.00

价格: ￥1600.00

PL.170 菌种冻存管Microbank
[试剂]
TRIzol Plus ...

2019-01-25

市场价: ￥3708.00

价格: ￥3152.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Plus RNA Purification Kit <img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///https://assets.thermofisher.com/tfs-assets/lsg/product-images/12183555_650x600.jpg-250.jpg"/> Catalog number: 12183555
[生化试剂]
TRIzol Reage...

2019-01-25

市场价: ￥2637.00

价格: ￥1978.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>  TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Reagent

[常用生化试剂]
头孢唑林钠Cefazol...

2019-10-29

市场价: ￥45.00

价格: ￥45.00

头孢唑林钠 <span style="font: 400 14px/24px arial,sans-serif; text-align: left; color: rgb(51, 51, 51); text-transform: none; text-indent: 2em; letter-spacing: normal; text-decoration: none; word-spacing: 0px; display: inline !important; zoom: 1; orphans: 2; font-size-adjust: none; font-stretch: normal; float: none; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255);">  <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">英文名称: <span style="text-align: left; color: rgb(0, 0, 0); text-transform: none; line-height: 1.5; text-indent: 0px; letter-spacing: normal; font-size: 14px; display: inline !important; float: none; background-color: rgb(255, 255, 255);" sans="" inline="">Cefazolin sodium salt
[常用生化试剂]
MES 2-(N-吗啡啉...

2019-10-29

市场价: ￥80.00

价格: ￥80.00

MES 2-(N-吗啡啉)乙磺酸 CAS号 145224-94-8  分子式 C6H13NO4S·H2O 分子量213.25
[生化试剂]
TRIzol Reage...

2019-01-25

市场价: ￥2637.00

价格: ￥1978.00

Invitrogen<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/>  TRIzol<img style="background:url(script/ueditor/lang/zh-cn/images/localimage.png) no-repeat center center;border:1px solid #ddd" src="script/ueditor/themes/default/images/spacer.gif" word_img="file:///C:/Users/DELL/AppData/Local/Temp/%7BD41FVA2GN7OA2X26ICDLK9.png"/> Reagent
[常用生化试剂]
D-潘糖 D-Pano...

2018-07-31

市场价: ￥1530.00

价格: ￥1530.00

 D-潘糖    潘糖     D-Panose      CAS：33401-87-5
[常用生化试剂]
Rifampicin利福...

2018-07-27

市场价: ￥460.00

价格: ￥391.00

利福平 Rifampicin 别名: 3-(4-甲基-1-哌嗪基亚胺甲基)利福霉素SV, 利米定, 甲哌利福霉素 CAS号:13292-46-1
[常用生化试剂]
利福平Rifampici...

2018-07-27

市场价: ￥120.00

价格: ￥120.00

利福平Rifampicin (Rifampin)    98%   Cas: 13292-46-1